ELISA kit for the quantitative determination of deoxynivalenol in cereals, soja, beer.

Overall process times were geared to requirements as defined by the USDA GIPSA test performance specifications.

Method: Competitive enzyme immunoassay

Detection limit: 0,5 ppb
Cereals (0,0125 ppb) - Beer (0,005) - Soy (0,0125)

Quantification limit: 2 ppb

Assay time: 60 min

Detection: 450 nm

Recovery rate:
Wheat (106%) - Corn (97%) - Beer (99%) - Soy (90%)

Standards: 00; 2; 8; 20; 40; 80 [ng/mL]
(ng/mL = 1 000 ppt)

Ref. Range: 0 – 80 ppb

Cross reaction:
- 15-Acetyl-DON 0.4%
- 3-Acetyl-DON 800%

Storage temperature: 2 - 8ºC

Format: Microplate - 96 wells

- Quantitative results based on superior 4PL curve fitting for calibration
- Microsoft Excel-based calculation & evaluation tool available
- Reagents (excl. wash buffer) ready-to-use
- 96 well format with breakable microwell strips
- Simple extraction procedures
- Incubation at room temperature
- High accuracy and reproducibility
- High stability; minimum shelf life 12 months

Cereals, soy and beer